engleski

Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones

Next generation sequencing of total RNA samples from four Croatian autochthonous grapevine varieties revealed the presence of a novel virus in two grapevine accessions. Complete genome sequence of a novel virus, tentatively named grapevine badnavirus 1 (GBV-1), was reconstructed from a de novo assembled contig. GBV-1 has a genome of 7, 145 nucleotides containing three ORFs with sequence similarity to other badnaviruses. In addition, several other viruses and viroids, including grapevine virus G, grapevine virus K/D, grapevine virus T, grapevine Roditis leaf discoloration-associated virus, grapevine yellow speckle viroids 1 and 2, and hop stunt viroid have been detected and identified for the first time in Croatian grapevines in the course of this study.

virus, grapevine, next generation sequencing

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