engleski

Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays

Safety assessment of nanoparticles (NPs) is a crucial during their design and development for biomedicine. As one of the prerequisite steps during this evaluation is the in vitro testing employing cell-based assays that are not always validated and well-adapted for NPs. Interferences with in vitro assays may arise due to nano-related optical, oxidative, fluorescent, surface and catalytic properties of NPs. Thus, proper validation of each assays system has to be performed for each NPs type. This study aimed to evaluate applicability of the most common in vitro cytotoxicity assays for safety assessment of up- and down-converting lanthanide-doped NPs. Conventional cell viability tests and fluorescence-based assays for oxidative stress response were selected to determine biological effects of up- and down-converting NPs to human brain cells. Comparison was performed with the known silver and iron oxide NPs for verification purpose. Both plate reader and flow cytometry measurements were examined. Obtained results indicated that both types of Ln-doped NPs interfered to much lesser extent than metallic NPs. In addition, the great potential of both up- and down-converting NPs for biomedicine has been manifested due to their biocompatibility and low toxicity.

photon up- and down-conversion ; nanoparticles ; interferences ; cell viability ; oxidative stress ; biocompatibility ; safety

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