Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Combined effect of extracellular Hsp70 and cigarette smoke extract on cytokine secretion from bronchial-epithelial NCI-H292 cell line (CROSBI ID 668215)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Grdić Rajković, Marija ; Hulina, Andrea ; Somborac Bačura, Anita ; Jelić, Dubravko ; Vukić Dugac, Andrea ; Rumora, Lada Combined effect of extracellular Hsp70 and cigarette smoke extract on cytokine secretion from bronchial-epithelial NCI-H292 cell line // The European respiratory journal. 2018. str. 3311-3311 doi: 10.1183/13993003.congress-2018.PA4250

Podaci o odgovornosti

Grdić Rajković, Marija ; Hulina, Andrea ; Somborac Bačura, Anita ; Jelić, Dubravko ; Vukić Dugac, Andrea ; Rumora, Lada

engleski

Combined effect of extracellular Hsp70 and cigarette smoke extract on cytokine secretion from bronchial-epithelial NCI-H292 cell line

Introduction: Cigarette smoke is one of the major risk factors for development of chronic obstructive pulmonary disease (COPD), which is characterized by chronic inflammation. Extracellular Hsp70 is increased in sera of COPD patients, and can act as damage-associated molecular pattern. Aims and objectives: We explored the pro-inflammatory effects of cigarette smoke extract (CSE) alone and in combination with recombinant human Hsp70 (rhHsp70) on bronchial-epithelial NCI-H292 cell line. Methods: NCI-H292 cells were treated with 2.5% or 15% CSE alone or in combination with 0.3, 1.0 or 3.0 µg/mL rhHsp70 for 24 h. IL-1α, IL-6 and IL-8 were determined in cell supernatants by ELISA method. Results: Compared to the non-treated cell, secretion of IL-1α, IL-6 and IL-8 was significantly increased after treatment with 2.5% CSE, but not in combination with rhHsp70. Combination of rhHsp70 and CSE resulted in different secretion of IL-1α, IL-6 and IL-8 compared to the cells treated with CSE alone, and theirinteractions were antagonistic. Concentrations of IL-1α, IL-6 and IL-8 were different after treatment of cells with 15% CSE or combination of CSE and rhHsp70 compared to the non-treated cells, but their concentrations did not differ after combined treatment compared to CSE alone.

chronic obstructive pulmonary disease ; extracellular Hsp70 ; cigarette smoke ; NCI-H292 cell line

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

3311-3311.

2018.

nije evidentirano

objavljeno

10.1183/13993003.congress-2018.PA4250

Podaci o matičnoj publikaciji

The European respiratory journal

European Respiratory Society

0903-1936

1399-3003

Podaci o skupu

28th International Congress of the European Respiratory Society (ERS 2018)

poster

15.09.2018-19.09.2018

Pariz, Francuska

Povezanost rada

Farmacija, Interdisciplinarne prirodne znanosti, Kliničke medicinske znanosti, Temeljne medicinske znanosti

Poveznice
Indeksiranost