engleski

Antibacterial efficacy of five bioactive root canal sealers against Enterococcus faecalis

Aim To determine the antimicrobial effect of five bioactive endodontic sealers against 48h old Enterococcus faecalis biofilms. Methodology Bacterial suspension with E. faecalis, which had been isolated from root canal with chronical apical periodontitis, was grown on cellulose nitrate membrane filters (Whatman GmbH, Germany) and placed on the Mitis Salivarius agar plates. After the incubation period (48h), the colonization was confirmed by scanning electron microscopy. Then, the membranes were divided into six experimental groups (n=8/each) according to the tested sealer: 1: TotalFill Bioceramic Sealer (BC Sealer, FKG, Switzerland) ; 2. BioRoot RCS (Septodont, Saint Maur Des Fosses, France) ; 3. MTA Fillapex (Angelus, Londrina, Brasil) ; 4. MTA Plus (PrevestDenpro, Bradenton, USA) ; 5. Apexit (Vivadent, Schaan, Liechenstein) ; 6. AH Plus (Dentsply, Konstanz, Germany). The sealers were prepared according to the manufacturers' instructions and approximately 40 µL of each sealer was placed on each membrane. After 30 minutes of exposure, sealers were carefully removed. The membranes were transferred to tubes containing sterile phosphate buffered saline (PBS) and agitated. After 10-fold serial dilution, the aliquots were plated on Mitis Salivarius agar plates. After 24 hours of incubation colony forming units (CFUs) were counted. Positive controls included membranes with E. faecalis and without sealer, and sterile membranes with applied sealer served as a negative contols. For the statistical analysis Mann Whitney U and Kruskal Wallis tests were used. Results All tested sealers reduced the number of bacteria significantly (p<0.005). The highest antibacterial effect was recorded in the Apexit group (p<0.005). There were no significant difference in the antimicrobial efficacy between other tested sealers (p<0.001). Conclusions All tested bioactive endodontic sealers showed significant and similar antimicrobial effect against E. faeacalis, however, Apexit was the most effective.

root canal sealers ; antibacterial ; enterococcus faecalis

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