engleski

Cytotoxicity and mutagenicity study of waste and purified water samples from electroplating industries prepared by use of ferrous sulfate and wood fly ash

Toxicological safety of the new purification method for electroplating waste waters (EWW) has been assessed. Method utilizes waste by-product ferrous sulfate and wood fly ash to scavenge heavy metals from EWW. Energy dispersive X-ray fluorescence (EDXRF) analysis of all samples met the law requirements for safe depositing of waste into the environment. The toxicity study consisted of determination of frequency of bacterial and human cell lines survival and the Ames assay (plate incorporation assay and the preincubation assay). Unexpectedly, data obtained indicate cytotoxicity and slight mutagenic potential of purified water. Ames assay showed that combination of alkalis and heavy metals present in the water purified with original fly ash and eluates of fly and waste ash were metabolically activated and conjugated with glutathione, resulting in new metabolites active and toxic for the cell. In order to reduce this effect pretreatment of fly ash (partial removal of highly soluble compounds) are necessary prior its usage for neutralization /coagulation/flocculation processes.

Ames test; HEp; HeLa; electroplating waste water; ferrous sulfate; wood fly ash

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