engleski

Association between CD30 expression and atopic dermatitis

Atopic dermatitis (AD) is chronic pruritic inflammatory skin disorder characterized by several clinical, immunological and biochemical alterations. Atopic dermatitis lesions contain TH2 like cells and TH2 cytokines release. It has been recently reported that CD30, a 120 kDa membrane-bound glycoprotein is an activation marker of T-cell clones, showing a TH2-related cytokine pattern of production.We investigate the presence of CD30+ cells in the lesional skin of patients, with atopic dermatitis compared with healthy skin as control.We obtained 30 biopsy specimens (3-4 mm punch biopsy) from various skin regions of 15 AD patients and 15 those with healthy skin. The immunohistochemistry peroxidase - antiperoxydase method with monoclonal antibodies was used for the analysis of parafine-embedded skin section biopsies, as well as anti-CD30, anti CD45RO+, anti-CD3 and anti-CD4 dilutions of antibody sera (Multi-link Swine anti Goat-Mouse- Rabbit Immunoglobulins biotimylated, Dako, Copenhagen, Denmark). The number of CD30+ cells in the whole biopsy tissue (x40) was counted. A semiquantative grading was used.Fifteen AD patients (7 women and 7 men) with acute AD and 15 healthy skin volunteers (12 women and 3 men) were included in the study. In all specimens of acute AD patients, most infiltrating cells were CD3+ T cells. In AD patients high CD30 expression was observed in a remarkable proportion of infiltrating cells. In the healthy skin CD30+ cells were very rare. Average CD30 expression was significantly higher in AD patients than in controls (p<0.01). Our analysis of CD30, CD3 and CD4 cells in skin biopsies of AD patients showed thus, CD30 expression in AD might be helpful in histologic differentiation of AD in accordance with the results obtained by Caproni et al. (1997) and Dumner et al. (1998). The results suggested a specific regulatory function of CD30+ T cells in acute atopic dermatitis lesions.

Atopic dermatitis; chronic pruritic inlammatory skin disorder; Th2-like cells; Th2 cytokines; CD30+ cells; CD3; CD4; skin biopsy

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