engleski

Resolved Fluorescence Emission Spectra of Prodan in Buffer/Ethanol Solutions

The fluorescence steady-state emission spectra of lipophylic fluorescence probe PRODAN in ethanol/water solvents of different concentrations (0.3, 0.9, 3 M ethanol) have been extensively studied and analytically described. After correction for background effects the complex experimental spectra were resolved into two Gaussian curves simulating two fluorescence transitions. The energy separation of (0.158 ± ; ; 0.001) eV was independent on ethanol concentration and temperature. For the decrease in solution polarity linear increase in fluorescence intensity accompanied with blue shift for both transitions were determined. The inclination of linear function for 25˚ C is steeper than the inclination of linear function for 37˚ C, and it is slightly more pronounced for  1 = 519 nm (37.5% increase) than for  2 = 555 nm (32.2% increase). The linear dependences of fluorescence's intensities on PRODAN concentration for all ethanol concentrations indicate that no PRODAN self quenching takes place even at higher PRODAN concentrations.

Prodan; ethanol; steady-state emission spectra

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