engleski

BPC 157 accelerates healing of transected rat quadriceps muscle

Stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, M.W. 1419 (PL-10, PLD-116, PL 14736 Pliva, Croatia)), accelerates healing of wounds, burns as well as transected Achilles tendon and shows strong anti-inflammatory effects. BPC is effective alone, without carrier, in trials for inflammatory bowel disease, wound treatment, locally or systemically, with no toxicity reported. We investigate whether this peptide improves healing and functional recovery of rat right quadriceps muscle following complete transection. After complete muscle transection, Wistar male rats received agents once daily, intraperitoneally, BPC 157 (10 µg/kg, 10 ng/kg or 10 pg/kg) or saline (5.0 mL/kg) (controls), first application 30 min following injury, last 24 h before sacrifice (at 4, 7, 14, 21, 28, 72 days post-injury). Assessment was functional, macroscopical and histological. Full assessment i.e., walking track analysis, biomechanical analysis, macroscopic assessment of bridging fragment between cut muscle ends, atrophy of distal part of transected muscle and microscopy (HE, Gomori silver stain, Van Gieson staining, vimentin and desmin immunostaining) reveals gastric pentadecapeptide BPC-157 induced healing and function recovery at all of the tested intervals. Eventually muscle regeneration was observed in BPC 157 treated animals in contrast to no regeneration, followed by complete atrophy of distal fragment evident in controls. In conclusion, these results indicate that this suitably stable gastric pentadecapeptide BPC-157 heals transected muscle.

BPC 157; transected muscle; healing; regeneration

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