engleski

MICA microsatellite polymorphism and astma in the Croatian children population

So far, there hasn’ t been a study, which would investigate the possibility of an association of the five known alleles of the MICA polymorphism with asthma in the Croatian population. Since atopic asthma is still intriguing, regarding its asociation with HLA region, we undertook this study to dissect possible relation. We have studied the MICA polymorphism in 122 children with asthma and 187 unrelated healthy controls. Patients were divided into 2 groups according to differential phenotypic expression of symptoms: group A- asthma only (53 patients) and group B-asthma with rhinitis (69 patiens). Forty three patients had low total IgE level (<400 kU/L), 39 patients had high total IgE (400 - 1000 kU/L) and 40 patients had very high total IgE (>1000 kU/L). All patients were Dermatophagoides pteronyssinus (Dp) sensitive ; 49 were Ambrosia elatior (Ae) and/or Dactylus glomerata (Dg) sensitive in adition. The MICA polymorphism was determined by PCR and electrophoresis on 6% polycrylamide gel in an automated sequencer (ALFexpress). Alleles were designated using the AlleleLocator program. Using appropriate statistical tests (Fisher exact test and Chi square), no significant association was found between any MICA allele and childhood asthma in our population. The distribution of MICA alleles demonstrated no statistically significant difference either when comparing group A and group B or when the patients were divided according to the total serum IgE or according to the sensitivity to Dp, Ae and Dg. Up to now our results indicate that MICA polymorphism does not play any role in developing asthma in the Croatian children population.

MICA microsatellite ; astma ; children ; Croatia

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