engleski

Effect of a Fat Free Diet on Fatty Acid Composition of Phospholipids in Rats' Erythrocytes

In this study, we have analyzed changes in fatty acid composition of phospholipids isolated from red blood cells (RBC) of rats fed fat free diet. Total lipids were extracted from RBC membranes, phospholipids were separated by column and thin layer chromatography and after a treatment with phospholipase A2 composition of fatty acids linked to sn-1 and sn-2 position of glycerol backbone was analyzed. Stereospecific analysis of fatty acids linked to sn-1 and sn-2 position of phosphatidylethanolamine fraction revealed statistically significant decrease in the ratio of linoleic and docosadienoic (C20:2) acid on sn-2 position, while sn-1 position remained unaffected. Similar results were obtained for mixed phosphatidylinositol + phosphatidylserine fraction where the ratio of oleic acid (C18:1, n-9) on sn-2 position was decreased. On sn-1 position, as well as in phosphatidylcholine fraction, no significant changes in fatty acid composition were found.

fat free diet; red blood cells; rat; phospholipid; fatty acid; phospholipase A2

nije evidentirano