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Measles virus proteome: virus proteins and host cell proteins

Measles virus (MEV) is an enveloped, nonsegmented, negative stranded RNA virus of the family Paramyxoviridae, subfamily Paramyxovirinae. MEV is the cause of measles, preventable by vaccination1. It is well established that during production various enveloped viruses incorporate host cell proteins, both inside the viral particle and in the lipid bilayer. Some host cell proteins might be included non-specifically, but there are also indications that some proteins are specifically taken up by the viral particles during direct interaction2-4. Some of these proteins even appear to play a role in the virus lifecycle. The presence of host cell proteins in viral particles is important for vaccine production because it implies that the vaccine does not only carry viral antigens but also host cell antigens. The aim of our research was to identify the proteome of MEV, consisting of 8 virus-coded proteins and to detect host cell proteins potentially present in virus preparations. MEV was grown in Vero cells, microfiltrated, and purified by hydrophobic interaction chromatography (HIC). Two obtained HIC eluates were concentrated by ultracentrifugation. Alternatively, MEV was purified only by ultracentrifugation. All the obtained samples of purified MEV were resolved by SDS- PAGE, protein bands were excised, in-gel digestion by trypsin was performed and the proteins were identified by MALDI-TOF/TOF-MS (peptide mass fingerprinting and peptide sequencing). Namely, the following virus proteins were identified: nucleoprotein, phosphoprotein, matrix protein, hemagglutinin, fusion protein and large protein. Actin, fibronectin and cyclophilin A were host cell proteins identified and confirmed in MEV samples. Different protein patterns were observed for differently purified samples indicating the importance of purification methods employed.

measles virus ; virus proteins ; host cell proteins ; MALDI-TOF/TOF

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